The Assessment of Toxicity Characteristics of Cellular Uptake of Paramagnetic Nanoparticles as a New Magnetic Resonance Imaging Contrast Agent

Nanoparticles are unique that enable many promising medical and technological applications intheir physical, and chemical properties. It is widely accepted that nanoparticles should bethoroughly tested for health nanotoxicity, but a moderate risk analysis is currently prevented by arevealing absence of mechanistic knowledge of nanoparticle toxicity. The purpose of this studywas to assess in-vitro cytotoxicity of Gadolinium oxide with diethylene glycol polymer (Gd2O3-DEG) and magneto liposome nanoparticles (MLNs) in Hepa 1-6 cell lines as models to assessnanotoxicity in-vitro. The effects of magnetic nanoparticles on these cell lines were evaluated by2light microscopy and standard cytotoxicity assays. The underlying interactions of thesenanoparticles with physiological fluids are key characteristics of the perception of their biologicalefficacy, and these interactions can perhaps be performed to relieve unpleasant toxic effects.Ourresults demonstrated that the Gd2O3-DEG and MLNs had significantlydifferent non-cytotoxiceffects. Our results suggest that these cell lines provide valuable models to assess the cytotoxicityof nanoparticles in-vitro. The results of the present study demonstrated that MLNs and Gd2O3-DEG with lower longitudinal relaxation time (T1) than Gadolinium Pentetic acid (Gd-DTPA) inHepa 1-6 cell lines are sensitive positive Magnetic Resonance Imaging (MRI) contrast agents thatcould be as attractive as candidates for cellular and molecular lipid content targets such as liverdiagnostic applications. These data reveal that MLNs is a useful positive contrast agent fortargeting and cell tracking. This will help to image of cells and special organs like liver that uptakesliposomal formulation very well.